Open AccessHeat inactivation of bovine serum used for blockade in immunoenzymatic assay is associated with spurious fall on the titers of anticardiolipin antibodies in primary antiphospholipid syndrome sera
Author(s) -
Nava Arnulfo,
Bañales José L.,
Reyes Pedro A.
Publication year - 1993
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860070209
Subject(s) - titer , antibody , cardiolipin , immunology , blockade , chemistry , anticardiolipin antibodies , medicine , biochemistry , phospholipid , receptor , membrane
Anticardiolipin antibodies (ACA) were evaluated using enzyme‐linked immunosorbent assay (ELISA) with and without heat inactivation of bovine serum used for plastic surface blockade. Untreated sera samples from primary antiphospholipid syndrome patients (PAS) and healthy blood donors (HBD) were tested. A significant decrease of ACA liters of PAS sera occurred with inactivated bovine serum blockade ELISA when compared with basal ELISA. In HBD sera there was no significant change. Probably, as happens with normal human serum, heating for normal bovine serum produces an increase in ACA titers. This bovine ACA may react with cardiolipin, and when human samples are added, they find antigen sites occupied, resulting in a spurious decrease of ACA titers. © 1993 Wiley‐Liss, Inc.