Open AccessStability studies of the components of a prototype penicillinase (β‐lactamase)‐linked ELISA kit
Author(s) -
Khatkhatay M. I.,
Desai M. P.,
Sankolli G. M.,
Pardhe D. K.,
Joshi U. M.
Publication year - 1993
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860070205
Subject(s) - chromatography , immunoassay , conjugate , pregnanediol , chemistry , substrate (aquarium) , reagent , antibody , hormone , medicine , biology , immunology , biochemistry , organic chemistry , mathematical analysis , ecology , mathematics
Penicillinase (β‐lactamase) enzyme‐linked immunosorbent assay (ELISA) for various reproductive hormones developed in the laboratory were found to have wide applicability in the fertility check clinic of the Institute. A need was thought to transform these assays into ready‐to‐use kit forms. Therefore, prototype ELISA kits for these hormones were developed and stability of the individual component was ascertained at various temperatures (room temperature, 37°C and 2‐8°C). Stability studies were conducted on previously validated assay for pregnanediol‐3α‐glucuronide (PdG). The studies showed that immunosorbents (antibody coated plates) are stable at room temperature for a period of 2 weeks, at 37°C for 1 week and at 2‐8°C for a period of 9 months when preserved after treatment with glycerol solution. The lyophilised conjugate, standard and immunoassay buffer, colour reagent, and its substrate were stable at 37°C up to 1 week and at room temperature up to 2 weeks and at 2‐8°C for a period of 6 months, during which the stability was studied. © 1993 Wiley‐Liss, Inc.