Open AccessFlow cytometric detection of recombinant human granulocyte‐colony stimulating factor binding to leukemic cells
Author(s) -
Tatsumi Noriyuki,
Yamane Takahisa,
Tsuda Izumi,
Okuda Kiyoshi,
Chaisiripoomkere Watana
Publication year - 1993
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860070203
Subject(s) - fluorescein isothiocyanate , flow cytometry , recombinant dna , acute myeloblastic leukemia , leukemia , granulocyte , granulocyte colony stimulating factor , microbiology and biotechnology , immunology , myeloid leukemia , biology , myeloid , chronic myelogenous leukemia , fluorescein , fluorescence , biochemistry , physics , genetics , chemotherapy , quantum mechanics , gene
To examine binding of recombinant human granulocyte‐colony stimulating factor to myeloid cells, the factor was labeled with fluorescein isothiocyanate, and incubated with blood specimens, which were then analyzed by flow cytometry. Neutrophils demonstrated an increased fluorescence, while lymphocytes were negative. These cell fractions were used as controls for cytometric binding assays of leukemic cells. Six patients with lymphocytic leukemia were negative in this assay. Ten of 15 patients with myelocytic leukemia were positive. All patients (n = 5) in myeloblastic crisis of chronic myelogenous leukemia were also positive. The flow cytometry results correlated well with the results of colony formation in response to granulocyte‐colony stimulating factor. The results indicate that our method is useful in predicting the susceptibility of leukemic cells to recombinant growth factors. © 1993 Wiley‐Liss, Inc.