Open AccessA flexible, efficient, checkerboard immunoblot system for the detection and semiquantitation of specific antinuclear antibodies
Author(s) -
Ritchie Robert F.,
Collins Marilyn,
Ledue Thomas B.
Publication year - 1992
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860060610
Subject(s) - anti nuclear antibody , antiserum , serial dilution , antibody , computer science , set (abstract data type) , medicine , immunology , pathology , alternative medicine , autoantibody , programming language
In this paper an uncomplicated method for the simultaneous detection and semiquantitation of 11 of the 12 commonly studied antinuclear antibodies (ANA) in a single run is described. This new application of checker‐board immunoblotting (CBIB) is based upon available technology and employs purified antigens which can be either purchased or produced in‐house. CBIB requires no electronic instrument, can be formatted to meet the needs of the user, is rapidly performed, and has acceptable labor and materials costs. Data on the use of the method to examine available reference antisera is presented. CBIB has also proven practical forthe clinical study of 18 sera, at two dilutions per membrane, for each set of specific antinuclear antibodies, also at two or more dilutions. © 1992 Wiley‐Liss, Inc.