Open AccessSimultaneous expression of type I procollagen mRNA and albumin in cirrhotic human liver
Author(s) -
Yamada Shuhei,
Kohi Takehiko,
Hata RyuIchiro,
Hirohashi Setsuo,
Kurata ShunIchi,
Senoo Haruki,
Sakakibara Kooko,
Hayashi Toshihko,
Nakane Paul K.
Publication year - 1992
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860060603
Subject(s) - in situ hybridization , microbiology and biotechnology , procollagen peptidase , immunohistochemistry , albumin , hepatic stellate cell , hepatocyte , messenger rna , dna , biology , gene , chemistry , biochemistry , in vitro , immunology , endocrinology
The gene expression of human type 1 procollagen was investigated in cirrhotic human liver by using in situ hybridization with nonradioactive DNA probes. Using in situ hybridizaiton can provide direct evidence for the cell type capable for type I collagen synthesis in tissues. T‐T dimerized DNA probes were used and DNAs hybridized in situ were detected immunohistochemically using specific antibodies against T‐T dimer. The data demonstrated that type I collagen is synthesized in hepatocyles and stellate cells in pseudolobules and in fibroblasts in Glissons capsules in cirrhotic human livers. we indicated hepatocytes morphologically and functionally by using immunohistochemical localization of albumin, which ws used as a marker of hepatocyte, since albumin is synthesized exclusively by hepatocytes. © 1992 Wiley‐Liss, Inc.