Open AccessG test, a new direct method for diagnosis of Candida infection: Comparison with assays for beta‐glucan and mannan antigen in a rabbit model of systemic candidiasis
Author(s) -
Miyazaki Takashige,
Kohno Shigeru,
Koga Hironobu,
Kaku Mitsuo,
Mitsutake Kotaro,
Maesaki Shigefumi,
Yasuoka Akira,
Hara Kohei,
Tanaka Shigenori,
Tamura Hiroshi
Publication year - 1992
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860060511
Subject(s) - mannan , systemic candidiasis , titer , latex fixation test , glucan , immunology , beta glucan , candida albicans , invasive candidiasis , microbiology and biotechnology , medicine , chemistry , biology , antibody , polysaccharide , biochemistry , corpus albicans , antifungal , fluconazole
An indirect method to measure betaglucan, a major structural component of yeast cell walls, is available, but has the disadvantage of requiring the combined use of two assays. Recent reports describe the fungal index, which measures the difference between the conventional limulus test, in which factors C and G react with endotoxin and beta‐glucan, and a new endotoxin‐specific test, in which only factor C reacts with endotoxin. The G test was developed as a direct method to measure beta‐glucan alone. In this study, the G test was examined in sera of rabbits with experimental systemic candidiasis, and compared with the fungal index and mannan assay. The G test showed positive in all rabbits with systemic candidiasis faster and with higher titers than with the fungal index. Three rabbits with fulminant systemic candidiasis showed higher levels of reactivity with the G test and the fungal index than two rabbits with mild reactions. Mannan was positive by at least one serum in four of five rabbits by the latex agglutination test, and there was a good correlation between these assays. The G test is a good serodiagnositc method for the detection of candidiasis. © 1992 Wiley‐Liss, Inc.