Open AccessEnzyme immunoassay and immunochemical characterization of pancreatic stone protein in human serum
Author(s) -
Katsuzaki Tomoyuki,
Tatemichi Noriyuki,
Takeichi Chikako,
Hayakawa Shinobu,
Hayakawa Tetsuo,
Shibata Tokimune,
Nakae Yasuyuki,
Naruse Satoru
Publication year - 1992
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860060308
Subject(s) - immunoassay , monoclonal antibody , chemistry , enzyme , chromatography , antibody , microbiology and biotechnology , coefficient of variation , biochemistry , biology , immunology
Monoclonal antibodies were raised against human pancreatic stone protein (PSP) and used for one‐step enzyme immunoassay (EIA). PSP‐S 2‐5 was employed as the standard in the assay. The assay's measurable range was 25‐1,500 ng/ml and within run coefficient of variation was 3.7‐6.4%. Analytical recovery of the assay was 101.5 ± 5.65% ( X ± SD). The results of experiments in which serum was fractionated by Mono S (cation exchange chromatography) suggested that most of immunoreactive material in human serum is PSP‐S 2‐5 . The EIA offers simple, rapid, and specific analysis of serum PSP level for clinical diagnosis. © 1992 Wiley‐Liss, Inc.