Open AccessDetection of hepatitis C virus (HCV) RNA in paraffin embedded tissue sections of human liver of non‐A, non‐B hepatitis patients by in situ hybridization
Author(s) -
Yamada Shuhei,
Koji Takehiko,
Nozawa Masayuki,
Kiyosawa Kendo,
Nakane Paul K.
Publication year - 1992
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860060109
Subject(s) - in situ hybridization , hepatitis c virus , virology , antibody , hepatitis c , rna , hepatitis , in situ , virus , microbiology and biotechnology , hepacivirus , biology , pathology , medicine , messenger rna , chemistry , immunology , gene , biochemistry , organic chemistry
Localization of hepatitis C virus (HCV) RNA was investigated by non‐radioactive in situ hybridization in human liver specimens of chronic non‐A, non‐B (NANB) hepatitis patients who were seropositive for antibodies to HCV (anti‐HCV). For in situ hybridization, T‐T dimerized synthetic oligodeoxynucleotide probes were used and DNAs hybridized in situ were detected immunohistochemically using specific antibodies against T‐T dimer. The data demonstrates that HCV‐RNA was localized in the cytoplasm of hepatocytes in human liver biopsies obtained from the patients with chronic NANB hepatitis seropositive for anti‐HCV.