Open AccessOxidative metabolism of polymorphonuclear leukocytes (PMN) in patients with IgA nephropathy
Author(s) -
Chen HungChun,
Tomino Yasuhiko,
Yaguchi Yutaka,
Fukui Mitsumine,
Yokoyama Kenichi,
Watanabe Asako,
Koide Hikaru
Publication year - 1992
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860060108
Subject(s) - oxidative metabolism , oxidative phosphorylation , nephropathy , metabolism , polymorphonuclear leukocyte , chemistry , immunology , granulocyte , biochemistry , medicine , endocrinology , diabetes mellitus , in vitro
Abstract The production of hydrogen peroxide (H 2 O 2 ) by neutrophilic polymorphonuclear leukocytes (PMN) after stimulation and the infiltration of PMN in glomeruli were determined in 20 patients with primary IgA nephropathy. The H 2 O 2 production of PMN after the stimulation was measured with a spectrophotometer using horseradish peroxidase as substrate. The results were as follows: (1) when PMN were pretreated with cytochalasine B, H 2 O 2 production after stimulation with heataggregated IgG (IgG) or serum‐treated zymosan (STZ) was significantly higher in patients with IgA nephropathy than in controls, and (2) there was an increased amount of PMN localized in glomeruli in patients with IgA nephropathy using immunofluorescence of monoclonal anti‐PMN antibody. It appeared that the increased renal infiltration of PMN which have a high potential for production of reactive oxygen species might induce the glomerular injuries in patients with IgA nephropathy.