Open AccessClinical evaluation of five therapeutic drugs using dry film multilayer technology on the opus™ immunoassay system
Author(s) -
Jandreski Mark A.,
Shah Jyoti C.,
Garbincius Jean,
Bermes Edward W.
Publication year - 1991
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860050610
Subject(s) - opus , immunoassay , computer science , medicine , chromatography , pharmacology , art , chemistry , immunology , art history , antibody
Five therapeutic drug assays, carbamazepine, phenobarbital, phenytoin, theophylline, and valproic acid, were evaluated using an automated random access system for performing thin dry film multilayer competitive immunoassays, the OPUS analyzer. All reagents for the therapeutic drug assays are contained in a coated multilayer film chip encased within a plastic bar‐coded test module and require no external or supplementary reagents. A serum or plasma sample is applied to the test module by the instrument and the fluorescence intensity from the module is measured after 6 minutes. We found the OPUS assays acceptable for clinical use. Within‐run coefficient of variations were 2.3–6.7%, between‐run, 2.9–7.6%. These methods correlated well with the Abbott TDx, having correlation coefficients of 0.92–0.97. Because of the instrument design and the stability of the reagents, weekly calibration is not needed and samples can be run immediately upon receipt in a random access fashion or can be batched together.