Open AccessSensitive competitive‐binding elisas for quantifying free kappa and lambda light chains in cerebrospinal fluid
Author(s) -
Stanescu Gabriel L.,
Swick Alan R.,
Tuohy Vincent K.,
Rudick Richard A.
Publication year - 1991
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860050310
Subject(s) - immunoglobulin light chain , polyclonal antibodies , kappa , cerebrospinal fluid , chemistry , antibody , antiserum , bence jones protein , microbiology and biotechnology , immunology , biology , pathology , medicine , mathematics , geometry
Simple, sensitive, and fully standardized solid‐phase enzyme‐linked competitive binding immunoassays to quantify free kappa and lambda immunoglobulin light chains are described. The assays were developed to measure the concentration of free light chains in cerebrospinal fluid (CSF), in part because elevated levels of free kappa light chains have utility as a diagnostic marker for multiple sclerosis (MS). Polyclonal rabbit antibodies raised against pooled Bence Jones proteins are bound to solid‐phase Staphylococcal protein A and used as the primary antisera in this assay. A pool of Bence Jones proteins isolated from the urine of 10 individuals with multiple myeloma are used as a biotin‐labeled ligand and to develop a standard curve. The assays as described are sensitive to the low nanogram range and are specific for free kappa or lambda light chains. The assays were found to have acceptable precision, and results correlated highly with concentrations determined using competitive‐binding radioimmunoassays previously described.