Open AccessPurification of soluble human t lymphocyte receptors for sheep erythrocytes
Author(s) -
Itano Eiko N.,
Ono Mario A.,
Sumigawa Mari,
Longo Leda M.,
Moura Nayla C.,
Mendes Nelson F.
Publication year - 1991
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860050303
Subject(s) - sephadex , receptor , chemistry , molecular mass , polyclonal antibodies , affinity chromatography , heterologous , size exclusion chromatography , lymphocyte , chromatography , microbiology and biotechnology , biochemistry , biology , antibody , immunology , gene , enzyme
Using a polyclonal heterologous anti‐soluble E‐receptor serum, we identified molecules of molecular weight circa 58,000 and 150,000 The soluble receptor molecule with molecular weight of approximately 58,000 (Rs1) was initially purified from supernatant of heated lymphocytes through chromatography on Sephadex G‐200 and/or DEAE‐cellulose. The soluble receptor molecule with molecular weight of approximately 150,000 (Rs2) is detected at high levels in the serum of patients with cancer and uremia. Rs1 and Rs2 present in serum from cancer patients were purified by chromatography on Sephadex G‐200 and by affinity chromatography using anti‐Rs1 IgG. 131 I‐labelled supernatant of heated lymphocytes binds to sheep erythrocytes and the elution and analysis of the molecules adsorbed showed bands of molecular weights approximately 58,000 and 150,000, confirming the receptor activity of these molecules.