Open AccessDetection of anti‐nuclear antibodies from patients with systemic rheumatic diseases by elisa using hep‐2 cell nuclei
Author(s) -
Jitsukawa Tomofumi,
Nakajima Satoko,
Usui Junko,
Watanabe Hiroshi
Publication year - 1991
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860050109
Subject(s) - antibody , titer , anti nuclear antibody , cytoplasm , nucleus , microbiology and biotechnology , antigen , immunofluorescence , cell nucleus , extractable nuclear antigens , autoantibody , indirect immunofluorescence , chemistry , population , immunology , biology , medicine , biochemistry , environmental health
A microtiter plate was coated with cell nuclei from HEp‐2 cells, and the stabilized nuclei were fixed with acetone for an enzyme‐linked immunosorbent assay (ELISA). Auto‐antibodies against nuclear antigens were detected from the sera of patients with various systemic rheumatic disease but not from healthy individuals by means of the nucleus ELISA procedure. Ninety‐one percent of anti‐nuclear antibody (ANA)—positive sera as demonstrated by immunofluorescence (IF) test were also judged as positive for ANAs by the nucleus ELISA and 5% of them as psudo‐positive. Patient's sera with homogeneous and fine‐speckled IF patterns displayed the highest ELISA titers. A large portion of ss‐A/Ro antigen is localized in the cytoplasm; therefore, anti‐ss‐A/Ro antibody was hardly detected by nucleus ELISA. The population of ANAs detectable by nucleus ELISA included anti‐ss‐B/La, anti‐DNA, anti‐histone, anti‐Sm, anti‐RNP, and anti‐scl‐70.