Open AccessTime‐Resolved fluorometric sandwich immunoassay for human growth hormone in serum and urine
Author(s) -
Hashida Seiichi,
Tanaka Koichiro,
Ishikawa Eiji,
Inoue Shinobu,
Hayakawa Kunio
Publication year - 1991
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860050107
Subject(s) - immunoassay , urine , chemistry , chromatography , detection limit , horseradish peroxidase , monoclonal antibody , liter , europium , antibody , endocrinology , enzyme , biochemistry , medicine , immunology , ion , organic chemistry
A specific and sensitive time‐resolved fluorometric sandwich immunoassay for human growth hormone (hGH) in serum and urine is described. A monoclonal anti‐hGH IgG 1 (5802)–coated polystyrene ball was incubated with serum or dialyzed urine and subsequently with europium ion‐labeled monoclonal anti‐hGH IgG 1 (5801). Europium ion bound to the polystyrene ball was measured by time‐resolved fluorometry. The detection limit of hGH was 0.3 pg/tube, which was 15‐fold higher than that by sandwich enzyme immunoassay using horseradish peroxidase as label. The assay range of serum hGH was 15–15,000 ng/liter using 20 μl of serum. The assay range of urinary hGH was 2–2,000 ng/liter using 150 μl of dialyzed urine. The detection limits of hGH in serum and urine by this immunoassay were satisfactory for measuring hGH levels in serum and urine of healthy children and in serum of healthy adults and higher levels but not in urine of healthy adults and in serum and urine of patients with hGH deficiency.