Open AccessHuman melanoma metastasis culture supernatant contains chemotactic factors for phagocytes
Author(s) -
Ventura M. T.,
Cerri A.,
Jirillo E.,
Antonaci S.,
Bonomo L.
Publication year - 1991
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860050105
Subject(s) - chemotaxis , melanoma , peripheral blood mononuclear cell , monocyte , metastasis , cell culture , immunology , cell , antibody , cancer research , chemistry , biology , microbiology and biotechnology , in vitro , medicine , cancer , biochemistry , receptor , genetics
Abstract Several findings point out that melanoma culture supernatants release soluble factors which modulate mononuclear cell chemotactic responsiveness. In this regard, either an enhancement or an impairment of chemotactic capacity has been found. Here, we provide evidence that supernatant recovered from human melanoma cell metastasis culture is able to trigger monocyte and polymorphonuclear cell chemotaxis. This activity is not affected by the presence of anti‐IL‐1 antibody during the assay. Further studies are in progress to isolate and characterize soluble factors involved in this activity.