Open AccessEvaluation of the stick enzyme immunoassay in caranx sp. and seriola dumerili associated with ciguatera
Author(s) -
Hokama Y.,
Asahina A. Y.,
Hong T. W. P.,
Shang E. S.,
Miyahara J. T.
Publication year - 1990
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860040509
Subject(s) - ciguatoxin , immunoassay , fish <actinopterygii> , fishery , monoclonal antibody , ciguatera , biology , zoology , antibody , immunology
The stick enzyme immunoassay (S‐EIA) using monoclonal antibody to ciguatoxin (MAb‐CTX) was used to examine clinically implicated fish and to pre‐screen two species of fish, Caranx sp. (ulua or jack) and Seriola dumerili (kahala or amberjack), supplied by sports fishermen. All of the clinically implicated fish from the Department of Health gave S‐EIA values ≥ 1.3. The Caranx sp. and Seriola dumerili considered safe (≤1.2 value) and consumed after testing gave no false‐negative results. The S‐EIA procedure using MAb‐CTX proved to be specific, sensitive, and simple to use in the laboratory. It also proved to be useful in screening two large carnivorous fish for ciguatoxin and related polyethers prior to consumption.