Open AccessAn elsa procedure to detect anti‐double‐stranded and anti‐single‐stranded dna binding antibodies in connective tissue disorders
Author(s) -
Orr Kenneth B.,
Foerster John
Publication year - 1990
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860040504
Subject(s) - dna , absorbance , antibody , microbiology and biotechnology , chemistry , connective tissue , conjugate , alkaline phosphatase , enzyme , double stranded , biology , biochemistry , immunology , chromatography , genetics , mathematics , mathematical analysis
A simple and sensitive enzyme‐linked immunosorbent assay (ELISA) is described for the measurement of antibodies to native DNA (double‐stranded) and to heat‐denatured DNA (single‐stranded) in sera of patients with connective tissue disorders. DNA bound to polyvinylchloride plates is incubated with serum samples, and antibodies to both single‐ and double‐stranded DNA are detected by means of goat antihuman IgG alkaline phosphatase conjugate. The binding in individual sera is expressed as binding units per milliliter and refers to the absorbance in relation to the absorbance value obtained with a set of standards. The various parameters of the ELISA assay are described, and the results are compared with results of the Crithidia and Farr DNA antibody assays. Two case reports are discussed.