Open AccessA rapid turbidimetric immunoassay for serum antistreptolysin‐o 2
Author(s) -
Otsuji Shogo,
Kamada Tetsuro,
Matsuura Tsuneaki,
Seki Masashi,
Tanaka Kiko,
Shibata Hideaki,
Honda Tetsuro
Publication year - 1990
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860040402
Subject(s) - immunoassay , chromatography , chemistry , standard curve , immunoprecipitation , antibody , immunology , medicine
We designed a rapid, quantitative turbidimetric immunoassay for serum antistreptolysin‐O (ASO), based on the immunoprecipitating velocity with purified streptolysin‐O (SLO), which can be measured turbidimetrically at 340 nm. The assay system involves polyoxy‐ethylene mono‐p‐nonylphenyl ether to accelerate and enhance the immunoprecipitation reaction. No antibody excess phenomenon is observed, up to 1,700 U/ml. Grossly icteric sera can be assayed. Patient sera gave acceptable results. Free cholesterol offers no interference. The standard curve is linear for values up to 800 U/ml. Results measured by the proposed method and those by the conventional microtitration method correlated well. Analytical recovery averaged 90.1% (80.3%‐102.5%). Within‐run and day‐to‐day CVs ranged from 2.1% to 3.5% and from 2.5% to 6.9% respectively. Working SLO is stable for at least 15 days at 4°C. The proposed method has proven to be superior to other available methods for measuring serum ASO.