Open AccessSimplified solid‐phase immunobead assay for detection of ciguatoxin and related polyethers
Author(s) -
Hokama Yoshitsugi
Publication year - 1990
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860040313
Subject(s) - ciguatoxin , immunoassay , chemistry , chromatography , antibody , fish <actinopterygii> , biology , immunology , fishery
This study examined the development of a highly simplified solid‐phase colored latex immunobead assay for the detection of ciguatoxin and related polyethers. This procedure was compared with the stick enzyme immunoassay previously reported. Chi‐square analysis of two separate experiments on 153 and 283 fish of various species gave χ 2 values of p < 0.001 and p < 0.005, respectively Agreement between the two procedures with 26 fish implicated in ciguatera poisoning was 100%. A preliminary assessment in the field showed encouraging results. The procedure appears to be simple and applicable to field use. Furthermore, this procedure should be applicable to other antibody‐antigen detections, especially low dalton determinations.