Multiparametric analysis of peripheral blood in the normal pediatric population by flow cytometry | Zendy

Wiener Doris | Zendy; Rowlands David T. | Zendy; Lowell Nancy | Zendy; Shah Shirish | Zendy; Malone John | Zendy; Lowitt Saul | Zendy

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Multiparametric analysis of peripheral blood in the normal pediatric population by flow cytometry

Author(s) -

Wiener Doris,

Rowlands David T.,

Lowell Nancy,

Shah Shirish,

Malone John,

Lowitt Saul

Publication year - 1990

Publication title -

journal of clinical laboratory analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.536

H-Index - 50

eISSN - 1098-2825

pISSN - 0887-8013

DOI - 10.1002/jcla.1860040305

Subject(s) - cd8 , flow cytometry , cd19 , cd3 , peripheral blood , lymphocyte , immunology , population , medicine , cytometry , reference values , peripheral blood lymphocyte , lymphocyte subsets , andrology , antigen , environmental health

Lymphocyte subset analysis was performed on 114 healthy children and 84 healthy adults. Samples were prepared by a whole blood lysis technique and analyzed by flow cytometry. The percentage and total number of CD3 +, CD4 +, CD8 +, and CD19 + lymphocytes were calculated for each of six age groups. A direct correlation with age was seen in the percentages of CD3 +, CD4 +, and CD8 + lymphocytes. The absolute number and percentage of total lymphocytes, the percentage and absolute number of CD19 + lymphocytes, and the absolute number of CD3 + lymphocytes decreased with age. No significant correlation with age was observed for white blood cells, the absolute number of CD4t and CD8+ lymphocytes, and the CD4 + /CD8+ ratio.

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