Open AccessMultiparametric analysis of peripheral blood in the normal pediatric population by flow cytometry
Author(s) -
Wiener Doris,
Rowlands David T.,
Lowell Nancy,
Shah Shirish,
Malone John,
Lowitt Saul
Publication year - 1990
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860040305
Subject(s) - cd8 , flow cytometry , cd19 , cd3 , peripheral blood , lymphocyte , immunology , population , medicine , cytometry , reference values , peripheral blood lymphocyte , lymphocyte subsets , andrology , antigen , environmental health
Lymphocyte subset analysis was performed on 114 healthy children and 84 healthy adults. Samples were prepared by a whole blood lysis technique and analyzed by flow cytometry. The percentage and total number of CD3 +, CD4 +, CD8 +, and CD19 + lymphocytes were calculated for each of six age groups. A direct correlation with age was seen in the percentages of CD3 +, CD4 +, and CD8 + lymphocytes. The absolute number and percentage of total lymphocytes, the percentage and absolute number of CD19 + lymphocytes, and the absolute number of CD3 + lymphocytes decreased with age. No significant correlation with age was observed for white blood cells, the absolute number of CD4t and CD8+ lymphocytes, and the CD4 + /CD8+ ratio.