Open AccessA dot‐elisa for the detection of igg antibodies to mumps and varicella viruses
Author(s) -
Leonardi Maria S.,
Gazzara Domenica,
Zummo Sebastiana,
Mastroeni Pasquale,
FattalGerman Michèle,
Bizzini Bernard
Publication year - 1990
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860040303
Subject(s) - nitrocellulose , virology , antibody , mumps virus , antigen , virus , biology , immunology , biochemistry , membrane
An enzyme‐linked immunosorbent assay using nitrocellulose strips (dot‐ELISA) for the routine laboratory detection of IgG antibodies to mumps and varicella viruses is described. The virus antigens are dotted onto nitrocellulose strips, and the dotted strips are incubated with the sera to be tested. The bound antibodies are revealed using enzyme‐labeled antihuman IgG antibodies. Reliable results are obtained when the assay is carried out at 37°C. The reported data indicate that the dot‐ELISA can reliably be used for the detection of IgG antibodies to mumps and varicella viruseis in human sera.