Open AccessA simple and rapid procedure for the direct detection of cytomegalovirus in urine samples
Author(s) -
Landini M. P.,
Guan M. X.,
Ripalti A.,
Lazzarotto T.,
La Placa M.,
Trevisani B.
Publication year - 1990
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860040302
Subject(s) - urine , cytomegalovirus , isolation (microbiology) , dna , virology , virus isolation , chromatography , biology , chemistry , virus , herpesviridae , microbiology and biotechnology , viral disease , biochemistry
We compared cytomegalovirus (CMV) isolation in tissue culture and viral DNA detection by DNA:DNA hybridization in 60 clinical urine samples concentrated by different procedures. Our results showed that urine concentration by filtration is the easiest and quickest procedure allowing further detection of CMV. With optimized working conditions, the CMV detection in urine can be done in 5 hr without requiring cell cultures or costly instruments.