Open AccessAn enzyme‐linked immunosorbent assay for the detection of antitetanus toxoid antibody using aluminum‐absorbed coating antigen
Author(s) -
Thiele G. M.,
Rogers J.,
Collins M.,
Smith D.,
McDonald T. L.,
Yasuda N.
Publication year - 1990
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860040210
Subject(s) - toxoid , tetanus , clostridium tetani , antibody , antigen , immunization , polystyrene , titer , immunology , microbiology and biotechnology , medicine , chemistry , virology , vaccination , biology , organic chemistry , polymer
Abstract An enzyme‐linked immunosorbent assay (ELISA) for the detection of antitetanus antibodies was developed using aluminum‐absorbed tetanus toxoid as the coating antigen. The assay was tested by measuring antitetanus antibody levels in serum obtained from subjects before and after immunization with the aluminum‐absorbed tetanus suspension. The specificity of the antibodies for tetanus antigen was tested both before and after antitetanus activity was removed with tetanus antigen–coated beads. Also, the activities of the sera were compared with those of a commercially available test. Our results indicated that the aluminum‐absorbed tetanus suspension can be coated onto standard polystyrene ELISA plates and used to measure antibody titers to tetanus toxoid.