Open AccessFunctional interactions between interleukin‐4, interleukin‐2, and tumor necrosis factor‐al for lymphokine‐activated killer cell generation
Author(s) -
Blay Jean Yves,
Branellec Didier,
Robinet Eric,
Gay Francoise,
Chouaib Salem
Publication year - 1990
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860040111
Subject(s) - lymphokine activated killer cell , lymphokine , interleukin 2 , tumor necrosis factor alpha , effector , immunology , interleukin , biology , cytokine , microbiology and biotechnology , cancer research , chemistry , immune system , t cell , interleukin 21
The purpose of the present study was to explore the interaction between interleukin‐2 (IL‐2), interleukin‐4 (IL‐4), and tumor necrosis factor‐a (TNF) on the differentiation of human large granular lymphocytes (LGL) into lymphokine‐activated killer cells (LAK). The data show that recombinant human IL‐4 (100‐1,000 U/ml) was able to induce the differentiation of human LGL into LAK effectors. The levels of the IL‐4‐induced cytotoxicity are significantly lower than those observed after stimulation of LGL by optimal doses of IL‐2. This LAK activity generation by IL‐4 was not associated with LGL proliferation. When TNF was added in LGL culture in the presence of sub‐optimal concentrations of IL‐4, the lytic capacity of the activated killer cells was significantly enhanced, suggesting an apparent synergy between these two factors. Most interestingly, our data indicate that exogenous TNF can partially overcome the known inhibitory effect of IL‐4 on IL‐Pinduced LGL differentiation into LAK effectors. These findings suggest a role for TNF in the process of LAK induction.