Open AccessEffects of radiolabelled monoclonal antibody infusion on blood leukocytes in cancer patients
Author(s) -
Gridley Daila S.,
Slater James M.,
Stickney Dwight R.
Publication year - 1990
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860040110
Subject(s) - monoclonal antibody , cd16 , cd8 , medicine , cancer , melanoma , flow cytometry , antibody , cd3 , lymphocyte , cd19 , immunology , natural killer cell , cytotoxic t cell , chemistry , cancer research , antigen , biochemistry , in vitro
This study was undertaken to investigate the effects of a single infusion of radiolabelled murine monoclonal antibody (MAb) on peripheral blood leukocytes in cancer patients. Eleven patients with disseminated colon cancer, malignant melanoma, or lung adenocarcinoma were infused with 111 In‐labelled anti‐ZCE 025, anti‐p97 type 96 9 , or LA 20207 MAb, respectively. Blood samples were obtained before infusion, immediately after infusion (1 hr), and at 4 and 7 days postinfusion. Flow cytometry analysis of CD3 + , CD4 + , CD8 + , CD16 + , and CD19 + lymphocytes showed increasing CD4:CD8 ratios in seven patients after infusion. This phenomenon was not restricted to antibody subclass or to type of cancer. Two of the remaining patients exhibited a marked post‐infusion increase in CD8 + cells. In all three patients with malignant melanoma, decreasing levels of CD16' lymphocytes were noted after infusion and natural killer cell cytotoxicity showed fluctuations which paralleled the changes in the CD16 + subpopulation. Oxygen radical production by phagocytic cells was markedly affected in three subjects. These results suggest that a single infusion of radiolabelled murine MAb may alter the balance of critical lymphocyte subpopulations and modulate other leukocyte responses in cancer patients.