Open AccessEffects of substance p on the spontaneous binding of salmonella minnesota r345 (rb) to human peripheral blood lymphocytes
Author(s) -
De Simone Claudio,
Misefari Aldo,
Antonaci Salvatore,
Maffione Angela B.,
Jirillo Emilio,
Covelli Vito
Publication year - 1989
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860030606
Subject(s) - salmonella , peripheral blood , cytotoxic t cell , cd8 , lymphocyte , bacteria , biology , microbiology and biotechnology , suppressor , chemistry , immune system , immunology , in vitro , biochemistry , gene , genetics
The effects of substance P (SP) on Salmonella minnesota R345 (Rb) binding to human peripheral blood lymphocytes (PBL) were evaluated. Two parameters of bacterial cytoadherence were considered, namely the binding lymphocytes (BL) and the number of bound‐bacteria/lymphocyte (BB). The results showed that SP inhibits both BL and BB in a significant manner. Furthermore, distribution of Salmonella binding to CD4+ and CD8 + lymphocytes was studied following SP pre‐treatment of lymphoid cells. This neuropeptide is able to hamper the bacterial cytoadherence to both T‐cell subpopulations and, in particular, the inhibitory effect on the T‐suppressor/cytotoxic subset was more pronounced. These findings are discussed in terms of SP intervention in the mechanism of host protection against invading microorganisms.