Open AccessUtility of the polymerase chain reaction in detection of gene expression in drug‐resistant human tumors
Author(s) -
Scanlon Kevin J.,
KashaniSabet Mohammed
Publication year - 1989
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860030512
Subject(s) - polymerase chain reaction , gene , microbiology and biotechnology , gene expression , drug resistance , biology , real time polymerase chain reaction , gene duplication , inverse polymerase chain reaction , genetics , multiplex polymerase chain reaction
Recently, an enzymatic amplification method, the polymerase chain reaction (PCR), was modified to amplify a sequence of a drug resistance gene. The PCR assay can confirm data achieved by conventional molecular biology techniques, while requiring less time and fewer patient cells. It can be quantitated for gene expression. The data generated make it possible to analyze m‐RNA expression in tumor samples without being limited to detecting only gene amplification in response to cancer chemotherapy. The PCR assay can be an effective device in the early detection of resistance to chemotherapy.