Open AccessEffects of storage conditions on lymphocyte phenotypes from healthy and diseased persons
Author(s) -
Claramae H.,
Miller H.,
Levy Norman B.
Publication year - 1989
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860030507
Subject(s) - flow cytometry , heparin , cd19 , lymphocyte , whole blood , immunology , medicine , cd3 , monoclonal antibody , andrology , cd8 , chemistry , antibody , immune system
Anticoagulant and room temperature storage of peripheral blood was evaluated in healthy and diseased persons (AIDS and CLL) for its effect upon lymphocyte phenotyping by flow cytometry. Whole blood was stored in either Li heparin, EDTA, or ACD for up to 96 hours. Aliquots at 5 time intervals were evaluated using CD3, CD2, CD4, CD8, and CD19 monoclonal antibodies. Blood from healthy donors stored in EDTA produced an apparent increase in T cells and decrease in B cells most evident by 96 hours storage. Blood from diseased persons stored in Li heparin up to 96 hours showed insufficient changes in phenotype to warrant a different clinical interpretation. We conclude that all three anticoagulants are adequate for short term (< 24 hr) room temperature transport and storage of peripheral blood for flow cytometry. Li heparin or ACD are more appropriate if prolonged transport times are anticipated.