Open AccessIgM to human cytomegalovirus: Comparison of two enzyme immunoassays and igm reactivity to viral polypeptides detected by immunoblotting
Author(s) -
Re M. C.,
Landini M. P.
Publication year - 1989
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860030307
Subject(s) - serology , cytomegalovirus , human cytomegalovirus , virology , antibody , enzyme , immunology , immunoglobulin m , cytomegalovirus infection , biology , microbiology and biotechnology , herpesviridae , medicine , virus , immunoglobulin g , viral disease , biochemistry
IgM detection by enzyme‐linked immunosorbent assay (ELISA) is the most used method of establishing a cytomegalovirus (CMV) infection during pregnancy. In this paper, we discuss the results obtained assaying 1,000 sera from pregnant women by two ELISA kits: a traditional indirect ELISA and a more recent IgM‐capture ELISA. All the sera that gave a positive ELISA value with one or both kits were further tested by immunoblotting (IB) to establish which CMV polypeptides were detected by IgM antibodies. From the results obtained, IgM‐capture ELSA seems less sensitive than indirect ELISA, but correlates better with serological evidence of active CMV infections as judged by typical IB profiles.