Open AccessSandwich immunoassay for the detection of lipoteichoic acid
Author(s) -
Tadler Monica B.,
Tice George,
Wos Susan M.
Publication year - 1989
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860030106
Subject(s) - lipoteichoic acid , immunoassay , chromatography , chemistry , computational biology , computer science , antibody , biology , bacteria , staphylococcus aureus , immunology , paleontology
A sandwich immunoassay has been developed for the detection of lipoteichoic acid (LTA), a major cell wall constituent of gram‐positive bacteria, from whole blood and ISOLATOR supernate. Monoclonal antibodies were produced to purified LTA from Streptococcus mutans , BHT and were further characterized for crossreactivity with gram‐positive and negative bacteria and for reactivity to substituted and unsubstituted LTA. Eight monoclonal antibodies were identified that reacted exclusively with gram‐positive bacteria. Those antibodies able to capture H‐LTA were chosen to develop a sandwich immunoassay. The assay has a sensitivity of 0.2 ng LTA/mL in PBS, 0.5 ng/mL in whole blood and 2.0 ng/mL in whole blood that has been processed through the ISOLATOR. Further development of this assay may lead to the rapid detection of LTA from body fluids.