Open AccessDot‐immunobinding assay with the globular domain of collagen type IV for antiglomerular basement membrane antibodies
Author(s) -
Weber M.,
Manns M.,
Meyer zum Büschenfelde K.H.,
Köhler H.
Publication year - 1988
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860020404
Subject(s) - basement membrane , nitrocellulose , antibody , type iv collagen , antigen , chemistry , dot blot , microbiology and biotechnology , goodpasture syndrome , membrane , laminin , glomerular basement membrane , pathology , immunology , medicine , biology , biochemistry , glomerulonephritis , kidney , extracellular matrix , dna
A dot‐immunobinding assay for the detection of antiglomerular basement membrane antibodies has been developed. The globular domain NC1 of basement membrane collagen type IV was used as antigen. The assay proved to be specific, sensitive, and reproducible. Circulating antibodies in each of 12 sera from patients with florid Goodpasture's syndrome could be demonstrated, whereas sera from patients with Goodpasture's syndrome in clinical remission and various control sera showed no reactivity. The advantages of the dot‐blot assay are: the usage of the purified Goodpasature target antigen NCI reduces unspecific binding of IgG; only minimal amounts of antigen are required to give a positive signal; evaluation of the assay is possible without expensive laboratory equipment; precoated nitrocellulose sheets can be stored for several months without loss of activity.