Open AccessDiagnosis of entamoeba histolytica in feces by ELISA
Author(s) -
Oliva Angel,
Herion Pascal,
Capin Ruth,
OrtizOrtiz Librado,
del Muro Ruben
Publication year - 1987
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860010403
Subject(s) - entamoeba histolytica , entamoeba , feces , entamoeba coli , monoclonal antibody , microbiology and biotechnology , amoebiasis , antigen , biology , human feces , antibody , immunology , helminths , ascaris lumbricoides
A diagnostic test for intestinal amebiasis was developed by using an ELISA that incorporated a well‐defined monoclonal antibody specific for a membrane antigen of Entamoeba histolytica . The ELISA was found to be sensitive and quite specific for E. histolytica , since there were no cross reactions with other parasites, especially Entamoeba coli , with which Entamoeba histolytica is often confused. In addition, this test detected not only E. histolytica trophozoites but also cysts. This technique will be of great value in the rapid and accurate diagnosis of E. histolytica in human fecal material.