Open AccessDiagnostic immunohistochemistry of lymphoma and related disorders: Practical aspects of frozen section technique and interpretation
Author(s) -
Robbins Bruce A.
Publication year - 1987
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1860010118
Subject(s) - immunoperoxidase , pathology , frozen section procedure , immunohistochemistry , lymphoma , lymphocyte , monoclonal antibody , medicine , biology , antibody , immunology
The diagnosis and classification of malignant lymphomas increasingly relies upon analysis of lymphocyte markers. Immunohistochemical techniques applied to frozen sections of lymphoid tissue have the advantage of allowing simultaneous evaluation of both immunologic phenotype and histologic/cytologic features. This paper describes the methodology currently employed in the Scripps institution for lymphocyte marker studies on tissue biopsies, including avidin‐biotin complex (ABC) immunoperoxidase and direct immunoperoxidase techniques. Emphasis is placed on technical aspects of the procedures and pitfalls in interpretation. A detailed listing of monoclonal antibodies against lymphocyte markers is provided, including technical features and important cross reactivities. The results of 60 cases analyzed over a one‐year period are presented. Essential immunophenotypic features of lymphoid hyperplasia, B‐cell lymphomas, T‐cell lymphomas, Hodgkin's disease, thymomas, and undifferentiated carcinoma are discussed.