Open AccessAsian multicenter trials on urinary type IV collagen in patients with diabetic nephropathy
Author(s) -
Tomino Yasuhiko,
Suzuki Shigenobu,
Azushima Chieko,
Shou Ichiyu,
Iijima Toshihiko,
Yagame Mitsunori,
Wang Li Ning,
Chen HungChun,
Lai KarNeng,
Tan Si Yen,
Kim Myung Jae
Publication year - 2001
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1026
Subject(s) - microalbuminuria , medicine , diabetic nephropathy , urinary system , nephropathy , urology , endocrinology , type iv collagen , diabetes mellitus , creatinine , gastroenterology , chemistry , biochemistry , laminin , cell
To investigate the changes of renal type IV collagen turnover in diabetic nephropathy, urinary type IV collagen was measured by a highly sensitive one‐step sandwich enzyme immunoassay (EIA). Urinary samples were obtained from 698 diabetic patients and 191 healthy adults. Among the patients, 264 had urinary albumin levels of less than 29 mg/g·creatine (Cr) (Stage I: normoalbuminuric stage), 169 had microalbuminuria from 30 to 299 mg/g·Cr (Stage II: microalbuminuric stage), 84 patients had macroalbuminuria of more than 300 mg/g·Cr and serum Cr of less than 1.1 mg/dl (Stage IIIA: macroalbuminuric stage without renal dysfunction), 97 had macroalbuminuria of more than 300 mg/g·Cr and serum Cr of more than 1.2 mg/dl (Stage IIIB: macroalbuminuric stage with renal dysfunction), and 84 had renal failure (Stage IV). The levels of urinary type IV collagen in Stages II, IIIA, IIIB, and IV were significantly higher than those in Stage I ( P < 0.0001). The level of urinary type IV collagen in Stage I (5.00 ± 0.23 μg/g·Cr; mean ± SE) was also higher than that in normal adults (3.44 ± 0.11 μg/g·Cr; mean ± SE). These levels increased gradually due to progression of the clinical stage of diabetic nephropathy. It appears that the levels of urinary type IV collagen can be a useful marker for detecting renal injuries in diabetes according to our Asian multicenter trials. J. Clin. Lab. Anal. 15:188–192, 2001. © 2001 Wiley‐Liss, Inc.