Open AccessSemiquantitative analysis of urinary low protein levels using silver dot blot assay
Author(s) -
Matsuda Kazuyuki,
Hiratsuka Nobuo,
Kurihara Yuriko,
Shiba Kiyoko
Publication year - 2001
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1022
Subject(s) - dipstick , chemistry , urine , densitometer , reagent , silver stain , chromatography , urinary system , dot blot , microbiology and biotechnology , biochemistry , biology , endocrinology , dna , physics , quantum mechanics
Abstract We designed a semiquantitative analysis of urinary low protein levels using silver dot blot assay. In this method, 3 μl of urine are blotted to one dot onto a cellulose acetate membrane, which is stained by a colloidal silver staining reagent, and the optical density of the silver stained urinary protein is measured at 500 nm using a densitometer. There was a good linearity between 2.5 mg/L and 100 mg/L and a gentle linearity between 100 mg/L and 200 mg/L, and the minimum sensitivity was 2.5 mg/L. This method is suitable for semiquantitative analysis of urinary protein levels less than 300 mg/L, which can not be determined precisely by dipstick. J. Clin. Lab. Anal. 15:171–174, 2001. © 2001 Wiley‐Liss, Inc.