Open AccessQuantitative PCR determination of human cytomegalovirus in blood cells
Author(s) -
Satou J.,
Funato T.,
Satoh N.,
Abe Y.,
Ishii K.K.,
Sasaki T.,
Kaku M.
Publication year - 2001
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.1014
Subject(s) - real time polymerase chain reaction , polymerase chain reaction , dna , microbiology and biotechnology , human cytomegalovirus , cytomegalovirus , biology , betaherpesvirinae , virology , herpesviridae , gene , virus , viral disease , genetics
We evaluated a rapid and sensitive method to determine human cytomegalovirus (CMV) DNA levels in blood cells using a quantitative polymerase chain reaction (PCR) technique. This method is based on real‐time detection of PCR using a dual fluorescence‐labeled probe and a sequence detector. Ten copies of CMV DNA were detected, when 1 μg of DNA from blood samples was used with this method, and a good correlation was obtained between increased concentrations of copy numbers calculated and measured copy numbers of CMV DNA (r = 0.999). Forty normal subjects exhibited no copies of CMV DNA. On the other hand, a 6‐month‐old girl tested positive for increased levels 4 weeks after liver transplant. This method is simple, accurate, and sensitive for the quantitative detection of CMV DNA in vivo, indicating possible applications for the diagnosis and monitoring of CMV infection. J. Clin. Lab. Anal. 15:122–126, 2001. © 2001 Wiley‐Liss, Inc.