Open AccessDevelopment of a dipstick dye immunoassay for diagnosing hydatidosis
Author(s) -
Sbihi Younes,
Gil José R.,
Alvarez Pedro A.,
Orduña Antonio,
RodríguezTorres Antonio,
Osuna Antonio
Publication year - 2003
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.10097
Subject(s) - dipstick , echinococcus granulosus , immunoassay , zoonosis , counterimmunoelectrophoresis , echinococcosis , medicine , pathology , antibody , immunology , biology , urine , zoology
We have developed a kit to diagnose hydatidosis, based on the detection of specific antibodies. This disease, caused by larvae of the cestode Echinococcus granulosus , is a zoonosis commonly found throughout the world. The diagnostic kit discriminates between positive and negative cases in a way that is easily interpreted, i.e., positive cases form a colored band on an inert substrate. The results show test sensitivity to be some 94.87% and specificity 85.71%, which is comparable to those of the ELISA technique. The high sensitivity and specificity of this test affords the great advantage of speed in diagnosing this parasitosis. No other equipment or procedure is required. J. Clin. Lab. Anal. 17:219–222, 2003. © 2003 Wiley‐Liss, Inc.