Open AccessEvaluation of a single‐step multiplex RT‐PCR for influenza virus type and subtype detection in respiratory samples
Author(s) -
Poddar Saibal K.,
Espina Rowena,
Schnurr David P.
Publication year - 2002
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/jcla.10036
Subject(s) - multiplex , multiplex polymerase chain reaction , virology , virus , immunofluorescence , biology , monoclonal antibody , influenza a virus , reverse transcription polymerase chain reaction , polymerase chain reaction , real time polymerase chain reaction , microbiology and biotechnology , antibody , gene , immunology , messenger rna , genetics
A single‐step multiplex reverse transcription‐polymerase chain reaction (RT‐PCR) for the simultaneous detection of influenza virus type and subtypes was evaluated for its use in clinical specimens. One part of each specimen was tested using an established standard culture and/or monoclonal antibody‐based immunofluorescence assay method, and the other part was tested by the multiplex RT‐PCR method for the presence or absence of the influenza virus, and its type and subtype. Sixty‐seven specimens were examined. The results revealed a strong agreement between the data obtained by the established method and those obtained by the multiplex RT‐PCR. J. Clin. Lab. Anal. 16:163–166, 2002. © 2002 Wiley‐Liss, Inc.