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Positively charged liposomes consisting of the KTTKS pentapeptide conjugated with rhodamine increase rhodamine toxicity in E. coli and zebrafish embryo
Author(s) -
Hsieh KaiHsuan,
Hsu ChihYing,
Hung IJu,
Yeh ChihLing,
Chen YauHung,
Cheng ChienChung
Publication year - 2021
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.202000358
Subject(s) - chemistry , liposome , rhodamine , toxicity , cell penetrating peptide , zebrafish , calcein , peptide , rhodamine b , biochemistry , biophysics , membrane , organic chemistry , fluorescence , quantum mechanics , photocatalysis , biology , gene , catalysis , physics
Abstract Liposomes composed of cell‐penetrating peptide derivatives increased transport across the cell membrane. Conjugating rhodamine to a cell‐penetrating peptide increased the toxicity of rhodamine in E. coli and zebrafish embryos. A similar total protein inhibition pattern with different intensities, indicating that the interaction pathways of the rho‐KTTKS‐CONH 2 monomer and liposomes were the same. It suggests that the rho‐KTTKS‐CONH 2 liposomes showed higher toxicity because better transport across the cell membrane increased the effective concentration inside cells. The staining of zebrafish embryos using rho‐KTTKS‐CONH 2 liposomes showed a longer retention time, suggesting that it can penetrate deeper tissues or organs in zebrafish.