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Novel thiazolidin‐4‐one clubbed thiophene derivatives via Gewald synthesis as anti‐tubercular and anti‐inflammatory agents
Author(s) -
Nayak Soukhyarani Gopal,
Poojary Boja,
Kamat Vinuta,
Puthran Divyaraj
Publication year - 2021
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.202000166
Subject(s) - chemistry , acetamide , thiophene , moiety , nuclear chemistry , mass spectrometry , hemolysis , stereochemistry , medicinal chemistry , organic chemistry , chromatography , immunology , biology
In aim of obtaining novel bio‐active compounds, a new series of 5‐(3,5‐difluorophenyl)‐2‐(2‐imino‐5‐arylidene)‐4‐oxothiazolidin‐3‐yl)thiophene‐3‐carbonitrile ( 6a–d ) and N ‐(3‐cyano‐5‐(3,5‐difluorophenyl)thiophen‐2‐yl)‐2‐(substitutedamino)acetamide ( 7a–f ) were synthesized via Gewald's method and characterized by 1 H/ 13 C nuclear magnetic resonance, Fourier‐transform infrared, mass spectrometry, and elemental analyses. All the newly prepared compounds were screened for their in vitro anti‐tubercular activity against virulent strain M. tuberculosis (H37Rv) at different concentrations ranging from 0.2 to 100 μg/ml. Most of the compounds were potent showing better inhibition at concentrations 6.25, 12.5, 25, 50, and 100 μg/ml. The anti‐inflammatory activity of the compounds was studied using inhibition of albumin denaturation technique. Tested compounds exhibited remarkable anti‐inflammatory activity. Hemolytic assay also confirmed that compounds are non‐toxic with a percentage hemolysis ranging from 1.81 to 14.94 at a concentration of 1 mg/ml. The results suggest that amino‐substituted acetamide and thiazolidin‐4‐one moiety, as well as the substitution pattern on the heterocyclic ring, have an effect on bioactivity.