Premium
A novel signal‐on fluorescent aptasensor for ochratoxin A detection based on RecJ f exonuclease‐induced signal amplification
Author(s) -
Wei Min,
He Xing,
Xie Yanli
Publication year - 2020
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201900423
Subject(s) - chemistry , aptamer , ochratoxin a , exonuclease , fluorescence , detection limit , signal (programming language) , exonuclease iii , chromatography , dna , microbiology and biotechnology , biochemistry , mycotoxin , dna polymerase , computer science , food science , gene , quantum mechanics , biology , physics , escherichia coli , programming language
Abstract This article describes a simple and homogeneous fluorescent aptasensor for the detection of ochratoxin A (OTA). With its high specificity and simplicity; RecJ f exonuclease is used to cleave DNA strand of the FAM‐aptamer/OTA complex and realize target recycling signal amplification. In order to avoid the loss of reaction system, magnetic beads (MBs) are added only once at the last experimental step. This proposed fluorescent aptasensor showed the higher sensitivity in the range of 0.1–100 ng/mL with LOD of 0.056 ng/mL, and the good selectivity against other interfering toxins. The feasibility of the prepared aptasensor was studied by detecting OTA in spiked liquor and cereal samples. The obtained average recoveries ranged from 92% to 115%. This study provides a promising application with convenience and rapidness in the aptasensor fabrication for food safety analysis.