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Biotinylated fluorescent probe for the specific and quantitative determination of blood glucose
Author(s) -
Lee FangHong,
Chew Chee Ying,
Hwu Jih Ru,
Tan KuiThong
Publication year - 2019
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201800293
Subject(s) - biotinylation , chemistry , fluorescence , avidin , quantitative analysis (chemistry) , chromatography , biotin , blood proteins , biochemistry , biophysics , physics , quantum mechanics , biology
The quantitative detection of biomarkers in protein‐rich samples using fluorescent probes is usually hampered by nonspecific fluorescence as a result of nonspecific probe–protein interactions. In this paper, we report a biotinylated fluorescent probe that is encapsulated in avidin protein, which can generate very specific fluorescence in blood serum by blocking out nonspecific dye–protein interactions. This approach was applied successfully to quantify glucose concentrations in blood serum.