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β‐ AgVO 3 Nanorods as Peroxidase Mimetic for Colorimetric Determination of Glucose
Author(s) -
Lu Junyu,
Wei Lianqiang,
Yao Dongmei,
Yin Xiuju,
Lai Hongfang,
Huang Xiuxiang
Publication year - 2017
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201700031
Subject(s) - nanorod , chemistry , abts , peroxidase , horseradish peroxidase , glucose oxidase , biosensor , detection limit , catalysis , nuclear chemistry , combinatorial chemistry , linear range , nanotechnology , chromatography , organic chemistry , enzyme , biochemistry , antioxidant , materials science , dpph
β‐ AgVO 3 nanorods have been demonstrated to exhibit intrinsic peroxidase‐like activity. The oxidation of glucose can be catalyzed by glucose oxidase ( GO x ) to generate H 2 O 2 in the presence of O 2 . The β‐ AgVO 3 nanorods can catalytically oxidize peroxidase substrates including o ‐phenylenediamine ( OPD ), 3,3′,5,5′‐tetramethylbenzidine ( TMB ), and diammonium 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonate) ( ABTS ) by H 2 O 2 to produce typical color reactions: OPD from colorless to orange, TMB from colorless to blue, and ABTS from colorless to green. The catalyzed reaction by the β‐ AgVO 3 nanorods was found to follow the characteristic Michaelis–Menten kinetics. Compared with horseradish peroxidase and AgVO 3 nanobelts, β‐ AgVO 3 nanorods showed a higher affinity for TMB with a lower Michaelis–Menten constant ( K m ) value (0.04118 mM ) at the optimal condition. Taking advantage of their high catalytic activity, the as‐synthesized β‐ AgVO 3 nanorods were utilized to develop a colorimetric sensor for the determination of glucose. The linear range for glucose was 1.25–60 μM with the lower detection limit of 0.5 μM . The simple and sensitive GO x ‐β– AgVO 3 nanorods– TMB sensing system shows great promise for applications in the pharmaceutical, clinical, and biosensor detection of glucose.