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Poly(cytosine)‐templated Silver Nanoclusters as Fluorescent Biosensor for Highly Sensitive Detection of Uric Acid
Author(s) -
Li Yanle,
Gong Nianchun,
Jiang Xi,
Zheng Xiaofang,
Wang Yaya,
Huan Shuangyan
Publication year - 2016
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201600121
Subject(s) - biosensor , chemistry , nanoclusters , detection limit , uric acid , fluorescence , cytosine , aptamer , nuclear chemistry , combinatorial chemistry , chromatography , biochemistry , organic chemistry , microbiology and biotechnology , dna , physics , quantum mechanics , biology
Uric acid (UA) is an important biomarker in urine and serum samples for early diagnosis. This study re‐ ports a fluorescent biosensor based on Poly(cytosine)‐templated silver nanoclusters (C‐Ag NCs) and uricase for the highly sensitive and fast detection of UA. The strong fluorescence of the C‐Ag NCs prepared from poly (cytosine) nucleotides templates could be sensitively quenched by trace amount of H 2 O 2 , which produced from oxidation reaction of UA catalyzed by uricase. This biosensor exhibits two linear ranges as 50 nM∼50 μM and 50 μM∼400 μM, with a detection limit of 50 nM. The sensitivity of the biosensor is considerably improved compared with the methods reported in the literature. Furthermore, the detection ability of uric acid in serum samples is confirmed and this C‐Ag NCs‐based uric acid biosensor shows good promise of practical application.