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Mechanistic Chemistry of Extraordinary Capacity of Salvianolic Acid B on Oxidatively‐damaged Mesenchymal Stem Cells
Author(s) -
Wang Tingting,
Li Xican,
Li Yunrong,
Wu Jianrun,
Huang Yuyin,
Wei Gang,
Chen Dongfeng
Publication year - 2016
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201600112
Subject(s) - chemistry , mesenchymal stem cell , radical , viability assay , antioxidant , oxidative stress , chelation , reagent , in vitro , stereochemistry , biochemistry , organic chemistry , microbiology and biotechnology , biology
As the main bioactive component of Chinese herbal medicine Danshen, salvianolic acid B (Sal B, or lithospermic acid B) was observed to maintain the viability of mesenchymal stem cells ( MSCs ) treated by Fenton's reagent in the study. Interestingly, at a higher concentration, Sal B could even increase the viability rate to 175.1%. In mechanistic analysis experiments, Sal B was found to resist DNA destruction by • OH radical, scavenge various radicals in vitro , reduce Cu 2+ → Cu + , chelate Fe 2+ to yield an absorption maximum at 710 nm. Based on these results, we concluded that, (1) Sal B can not only protect MSCs against • OH ‐induced damages, but also promote their proliferation. This extraordinary capacity makes Sal B an ideal candidate in MSCs transplantation especially when MSCs are polluted by iron‐overload or other oxidative stress factors and, can partly be responsible for the versatile properties of Sal B in pharmacology. The possible mechanisms of its protective effect are hypothesized to include Fe 2+ chelating, and direct radical scavenging which is involved in electron transfer ( ET ) or hydrogen atom transfer ( HAT ) from the catechol moieties. Its proliferation‐promoting effect is presumed to be from its ester group, carboxylic group, or benzofuran ring.