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Surface Plasmon Resonance‐based Inhibitive Immunoassay Coupled with Dummy Template Molecularly Imprinted Polymer Solid Phase Extraction for On‐line Analysis of Trace Clenbuterol
Author(s) -
Tang Pingping,
Huo Jichuan,
Zhang Hongping,
Lei Hong,
Lin Xiaoyan
Publication year - 2014
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201400153
Subject(s) - chemistry , detection limit , molecularly imprinted polymer , surface plasmon resonance , chromatography , analytical chemistry (journal) , solid phase extraction , adsorption , freundlich equation , calibration curve , extraction (chemistry) , biosensor , nanoparticle , nanotechnology , materials science , biochemistry , organic chemistry , selectivity , catalysis
A new indirect inhibitive immunoassay using surface plasmon resonance (SPR) coupled with molecularly imprinted polymer (MIP) was developed and applied for the analysis of trace clenbuterol (CL). A MIP coating using phenylephrine as the dummy template was synthesized in a flexible quartz capillary (30 cm×0.25 mm i.d.) by in situ polymerization technique, which then was used as the online solid phase extraction (SPE) tube before SPR detection. The thickness of the coating was 198 nm on average, illustrated by scanning electron microscope (SEM) image. The mechanism for adsorption of CL on dummy template MIP was found to be a Freundlich isotherm and pseudo‐second‐order model. The immunoassay was conducted on BIAcore 3000 biosensor automatically without pre‐treatment. The calibration curve was generated by linear fit in the range of 0.1–10 ng L ‐1 and 10–100 ng L ‐1 . The detection limit was 0.1 ng L –1 , which is super sensitive. This method was directly applied for the analysis of real‐world samples without pretreatment.