Premium
Electrochemical Studies of Anticancer Herbal Drug Sophoridine and Its Interaction with DNA
Author(s) -
Liu Hui,
Zhao Dongwang,
Zhao Aijuan,
Zou Keke,
Li Tiesheng,
Wu Ynagjie
Publication year - 2014
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201300584
Subject(s) - chemistry , electrochemistry , dna , drug , stoichiometry , anticancer drug , phosphate buffered saline , ic50 , pharmacology , combinatorial chemistry , in vitro , biochemistry , chromatography , medicine , electrode
Sophoridine is a new anticancer herbal drug with remarkable antitumor effect and lower toxicity. In this paper, the electrochemical investigation of sophoridine and its interaction with DNA is reported. In 0.1 mol L −1 phosphate buffer solution (pH 7.0), sophoridine exhibited excellent electrochemical activity. In the presence of DNA, the peak current of sophoridine decreased in a quantitative fashion and the peak potential shifted to a more positive potential value. It suggests the dominance of intercalative interaction. Importantly, as to the interaction between sophoridine and DNA in solution, characteristic parameters such as the binding stoichiometry and association equilibrium constant according to the Hill model for cooperative binding have been determined.