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Chiral Separation of Ofloxacin Enantiomers by Microchip Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection
Author(s) -
Chen Bing,
Zhang You,
Xie HaiLing,
Chen QiuMei,
Mai QiHong
Publication year - 2014
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201300500
Subject(s) - capillary electrophoresis , chemistry , enantiomer , ofloxacin , chromatography , calibration curve , detection limit , analytical chemistry (journal) , conductivity , stereochemistry , biochemistry , ciprofloxacin , antibiotics
We describe a chiral separation method for ofloxacin enantiomers, levofloxacin and dextrofloxacin by microchip capillary electrophoresis with capacitively coupled contactless conductivity detection. The running buffer included 1 mmol L −1 MES and 1 mmol L −1 Tris (pH 8.0) with a separation voltage of 1.5 kV and an injection time of 10s. Under these conditions, the enantiomers were completely separated within 1 min. The linear calibration curves were A = 5.76 c — 0.00587 for levofloxacin and A = 5.41 c — 0.00551 for dextrofloxacin, in which the linear concentration of the components all ranged from 0.05 to 0.15 mg mL −1 (regression coefficients were both 0.9996). The limits of detection (S/N = 3) were, respectively, 18 and 21 μg mL −1 . The relative standard deviations of migration time were both 2.0% ( n = 6). The relative standard deviations of peak area were 3.4% ( n = 6) for levofloxacin and 4.0% ( n = 6) for dextrofloxacin. The effects of some factors on resolutions, such as separation voltage and injection time, concentration of running buffers, were studied. The method was simple, rapid, high‐efficient. Furthermore, the method could be applied to the chiral separation of the product containing these enantiomers, such as Ofloxacin Eye Drops.