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Synthesis and DNA Cleavage Activity of Piperazine Containing Guanidinoethyl and Hydroxyethyl Side Arms
Author(s) -
An Dong,
Ye Zhiwen
Publication year - 2014
Publication title -
journal of the chinese chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.329
H-Index - 45
eISSN - 2192-6549
pISSN - 0009-4536
DOI - 10.1002/jccs.201300321
Subject(s) - chemistry , phosphodiester bond , piperazine , cleavage (geology) , dna , catalysis , stereochemistry , radical , hydrochloride , organic chemistry , biochemistry , rna , geotechnical engineering , fracture (geology) , engineering , gene
According to active groups synergetic catalysis principle, a novel phosphodiester receptor 1‐( N ‐guanidinoethyl)‐4‐( N ‐hydroxyethyl)‐piperazidine hydrochloride was firstly synthesized and the preliminary studies of its DNA cleavage activity. It is characterized and confirmed by methods as 1 H NMR and 13C NMR. A “couple hardness with softness” piperazidine is designed to connect guanidinium group and hydroxyl group; The reserch was showed that the best cleaving conditions was 7.2 in pH; Cleaving DNA by the compound reaction was oxidation process that were proved through the free radicals quenches experiments; The compound can cleaved the pUC 19 DNA by phospholipid transferance was proved by BDNPP.