Fluorescent Dye Incorporated Magnetic/Silica and Fluorescent Silica Nanoparticles Based Myoglobin Detection from Whole Blood Samples

The anti myoglobin conjugated iron oxide/ru(bpy) 3 2+ /silica, anti myoglobin conjugated rhodamine 6G/silica particles were prepared, and used for myoglobin detections. The anti myoglobin conjugated iron oxide/ru(bpy) 3 2+ /silica particles were mixed with myoglobin in the micro centrifuge tube. After 10 min, the magnetic separators were introduced to catch the myoglobin‐captured anti myoglobin/iron oxide/ru(bpy) 3 2+ /silica particles from the homogenous solutions. For sandwich assays, the anti myoglobin conjugated rhodamine 6G/silica particles were incubated. The concentration of myoglobin was quantified based on anti myoglobin conjugated rhodamine 6G/silica particles. The calibration curve of the myoglobin showed the linear range to be between 1 × 10 −12 and 1 × 10 −10 (M) (R 2 = 0.9944). The minimum detectable concentration was 17.6 pg/mL. The fluorescence method offered the best way to determine myoglobin with a total analysis time of less than 30 min.